指狀絲狀蟲在臺灣之分佈情況及免疫反應

外文標題: 
The Distribution Of Setaria Digitata In Taiwan And Its Immune Response
校院系所: 
國立台灣大學 獸醫學系研究所
指導教授: 
費昌勇
出版年份: 
1992年
主題類別: 
摘要: 

 指狀線狀蟲(Setaria digitata)於臺灣之分佈情形乃屬於全島性的,遍及全省之大多縣市,共計調查953頭牛,於腹腔中可發現蟲體者為195頭,陽性率達20%。將蟲體以外科手術植入天竺鼠及兔之腹腔後,蟲體在2至3週完全吸收,血清抗體可與蟲體抽取液(抗原)在凝膠沉降反應中有顯著反應。其沉降線在天竺鼠及兔血清對蟲體抗原之反應為相同(identical);但此二者與陽性牛之抗體,對蟲體抗原之反應則均為部份相同(partical - identical)。於酵素連接免疫轉印法(Enzyme - linked immuno - electro - transfer blotting, EITB)之分析顯示天竺鼠及兔之抗體反應較牛之抗體反應複雜(反應band較多)。天竺鼠、兔及牛三者共同出現之反應在分子量41.5k及47.7k兩處。   病理切片上顯示腹腔之蟲體結節形成早期蟲體子宮部之週圍嗜嗜中性球及酸性球之浸潤,蟲體外團則由多數單核球形成淋巴節樣結構。在中期蟲體有部份缺損,炎症反應明顯,可見許多巨嗜球,網狀內皮細胞,及類上皮細胞浸潤。晚期則見蟲體鈣化,週圍有類上皮細胞浸潤及巨大細胞浸潤;淋巴細胞消失,外圍由許多結締組織包被。   就免疫染色而言,將蟲體切片以免疫染色法(Immuno - peroxidase staining )染色,顯示指狀絲狀蟲之重要抗原應為子宮內之成份。亦即當蟲體釋放出微絲蟲時,所伴隨釋出之體液。

外文摘要: 

 In Taiwan, the distribution of Setaria digitata is islandwide and spreading over most of counties. We investigated 953 calves and found worms in the peritoneal cavities of 195 calves. The positive rate for the infection of Setaria digitata is up to 20% After surgical transplanting the worms into the peritoneal cavities of guinea pigs and rabbits, the worms were completely absorbed within 2 - 3 weeks. The sernm antibodies have prominent reactions with worm extract (antigens) in agar gel immunoprecipitation. The precipitation lines of the sera of guinea pigs and rabbits with worm antigens are edentical, but the precipitation of these two sera and bovine serum with worm antigens are partial - identical. In the analysis of enzyme - linked immunoelectrotransfer blotting, EITB, the antibodies of guinea pigs and rabbits have more complicated reaction than the antibodies of cattles (more reacting bands). The common two reaction sites of guinea pigs, rabbits and cattles are of molecular weight 41.5 K and 47.7 K.    Histopathologically, the early worm nodnles were infiltrated with eosinophils and neutrophils around worm uterus, and the worms were surrounded by many mononuclear cells and formed lymphoid nodule structure. In the middle phase, the worms were partiaily damaged, and the inflammatory respose became more obvious, Macrophages, reticuloendothelial cells and epithelioid cells were seen during this period. In the late phase, worms were calcified and infiltraied by epithelioid cells and giant cells, hymphooytes were disappeared, and worms were surrounded by many connective tissues.   By immunoperoxidase staining, it appears that the most important antigen components of Setaria digitata were the contents in worm uterus. That is the body fluid released while female worms releasing microfilaria.